Técnica de aspiração folicular guiada por ultrassom (ovum pick up – opu) em bovinos e equinos: revisão de literatura.

Abstract

The national cattle raising obtains continuous and rapid economic development with the advancement of reproductive biotechnology techniques. These allow the possibility of

increasing the production of animals that have characteristics of high interest. Ultrasound- guided follicular aspiration (Ovum Pick Up - OPU) is a widely used biotechnique in large

animals with the aim of genetic improvement in the herd. This consists of obtaining oocytes from females with high added value, so that they can be used for the production of embryos in the laboratory and for the rapid dissemination of specific genetics. Mares and cows are the animals in which the technique has been most explored and has generated excellent results. The steps pertaining to the in vivo follicular aspiration technique consist of selection of oocyte donor females and prior preparation, organization of material and equipment on the day of aspiration, anesthesia and asepsis, aspiration procedure, post aspiration care, and oocyte tracking and selection collected. Most of these steps are performed in both horses and cattle. However, in view of the physiological and reproductive particularities between these two species, the steps take place in different ways. This work aims to describe the reproductive biotechnique of OPU, present the stages of the process, highlighting the methodological differences that are adopted for the use of this technique in both cattle and horses.