GRAD CMZL- Cursos de Graduação

URI permanente desta comunidadehttps://ri.ifam.edu.br/handle/4321/175

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Análise histológica do segmento sexual renal de suaçuboia (Corallus Hortunalus, Squamata: Boidae)
(2020-12-11) Pinheiro, Yago Nazaré Araújo,; Oliveira, Maria Ermelinda do Espírito Santo; Amaral, Rodrigo de Souza; Amaral, Rodrigo de Souza; Santos, Felipe Faccine dos; Silva, Kalena Barros da
Corallus hortulanus is a snake with wide geographical distribution and valid ecological importance. However, information about their reproductive biology is scarce and most of the data obtained is from snakes in captivity. Thus, the work aims to describe the histological aspects of the sexual segment of the kidney (SSK), a specialized part of the kidneys. Therefore, histological analysis and measurement of SSK were performed from 12 specimens of Corallus hortulanus collected in different months of the year. The histological aspects of the SSK observed were similar to those described for other species of snakes and the tubular segments had an average diameter of 76.8 ± 25.6 μm. However, a great variation was observed in the morphological aspects (hypertrophy, presence and disposition of granules) and in the diameter of the tubular segments among the individuals analyzed, apparently related to reproductive seasonality. The results obtained in this study contribute to a greater understanding of the reproductive aspects of C. hortulanus and the possible seasonal variations should be better investigated. Keywords: SSK. Histology. Reproduction. Snake. Amazon Tree Boa.
Avaliação histológica do testículo e vesícula seminal de peixe-boi da Amazônia ( trichechus inunguis)
(2022-01-14) Viana, Karoline Araújo; Amaral, Rodrigo de Souza; http://lattes.cnpq.br/3589101457101968; Amaral, Rodrigo de Souza; http://lattes.cnpq.br/3589101457101968; Santos, Felipe Faccini dos; CV: http://lattes.cnpq.br/2863984157626236; Kokubun, Hanna Sibuya; e CV: http://lattes.cnpq.br/2912384036403340
The Amazonian manatee (Trichechus inunguis) belongs to the order Sirenia and it is an endemic aquatic mammal of the Amazon basin. Several studies on the biology of this species have been developed, however, the morphological aspects of the male reproductive system are not described yet. Therefore, the aim of this study was to describe the histological characteristics of the testis and seminal vesicle of this species. Samples from the reproductive system of 11 Amazonian manatees of different age classes were used, previously fixed in 10% formalin and deposited at the Aquatic Mammals Laboratory/INPA. The material was morphologically evaluated, and fragments were processed by traditional histological techniques, stained with hematoxylin and eosin and analyzed by light microscopy. The sections were photographed and its structures evaluated for morphology and morphometry. In general, the morphology of the reproductive system of the male Amazonian manatee was similar to that reported for other sirenian species. Furthermore, it was possible to differentiate sexually mature and immature animals based on their morphological aspects. The data obtained also corroborate the estimated age of sexual maturity for the species. This is the first report of histological evaluation of testicle and seminal vesicle of Amazonian manatee and, based on the results obtained, further studies should be carried out in order to understand the morphological variations observed in the analyzed adult individuals. Keywords: Reproduction. Histology. Sirenians.
Castração cirúrgica em cadelas: é realmente necessária?
(2021-04-23) Chagas, Anna Victoria Chacon Barreto Silva.; Amaral, Rodrigo de Souza; Amaral, Rodrigo de Souza; Tonin, Alexandre Alberto; Vieira, Flavia Volpato
Surgical castration in bitches is possible through some surgical techniques, such as ovariectomy, ovariosalpingohisterectomy, hysterectomy and even newer techniques, such as video-assisted castration. These procedures involve several benefits that are currently well discussed, including population control, behavioral changes, prevention and treatment of neoplasms and disorders of the female reproductive tract. However, there are also risks involved in the practice of sterilization, and these can compromise the lives of these animals in the short, medium and long term, and are closely linked to the applied technique, as each method has a different complication frequency. Castration can be done prepubertal or postpubertal, but the two options involve benefits and disadvantages that need to be balanced. Unfortunately, the disadvantages of this procedure are rarely discussed, and as it is an irreversible procedure, it should be applied more carefully, individualizing each case. Therefore, this review presents some applied techniques, along with the benefits and risks of its use. Keywords: Sterilization. Techniques. Bitches. Benefits. Disadvantages.
Cuidados iniciais com o neonato canino-revisão de literatura
(2019-12-05) Esteves, Isis Mônica Fontes; Amaral, Rodrigo de Souza; http://lattes.cnpq.br/3589101457101968; Amaral, Rodrigo de Souza; http://lattes.cnpq.br/3589101457101968; Vieira , Flavia Volpato; http://lattes.cnpq.br/1627861597325307; Hoffmann, Daniel José; CV: http://lattes.cnpq.br/4603328886760993
Neonatology is defined as the science responsible for the study concerning newborns. The canine neonatal period comprises the first two weeks of life of the puppy and this time requires specific care due to its fragility and particularities. The neonatal phase is full of adaptations that occur concomitantly with the development of vital functions not performed during intrauterine life. During birth the newborn is subjected to stress when moving from a liquid, closed and stable temperature environment to a dry, open and variable temperature environment, being forced, even under the best conditions, to undergo circulatory, respiratory, metabolic and other changes. Immunological So many changes make neonatal physiology different when compared to adult canine and require specialized care, as the mortality of puppies in veterinary medicine is high compared to human medicine. The bitch's health before pregnancy may reflect directly on embryonic, fetal and neonatal survival. Therefore, the female should be followed before pregnancy, undergoing pre-pregnancy examinations and throughout prenatal follow-up. Given the above, this paper aims to perform a literature review on the care to which the canine newborn should be submitted, specifically considering it’s particular physiology, some of the pathologies that may affect it and the integrity of maternal health to ensure its viability to the world. extrauterine.
Doença renal crônica em felinos - revisão de literatura.
(2022-11-24) Prado, Maisa Renata Rosas do; Vieira, Flávia Volpato; Vieira, Flávia Volpato; Amaral, Rodrigo de Souza; Pereira, Larissa Quinto
Chronic kidney disease (CKD) is more commonly seen in cats of advanced age, being one of the most common causes of mortality in these animals. CKD is the result of a gradual loss in the number of nephrons leading to impairment of metabolic, excretory, endocrine and renal functions. The etiology of the disease is multifactorial and can be stimulated by numerous causes and from different origins. Polyuria and polydipsia can be the first clinical signs, however, at the beginning of the disease, the clinical manifestations can be nonspecific, so when the disease is discovered, it is usually in advanced stages, since it is difficult to identify it early. The assessment of the glomerular filtration rate (GFR) is the most efficient method for diagnosing CKD, however, it is impracticable to be performed in the clinical routine, therefore, the diagnosis is based on renal biomarkers such as serum creatinine, SDMA, calcium and phosphorus, urea and others. The International Renal Interest Society (IRIS) establishes that after the animal is diagnosed, it must be correctly staged based on creatinine, SDMA, proteinuria and blood pressure. The importance of correct staging is to provide the animal with the correct treatment based on the stage and the clinical manifestations presented, because, as it is an irreversible and progressive disease, it does not have a cure, its treatment is carried out based on the clinical manifestations and to give a better quality of life for the animal. Because it is a difficult disease to be identified early and is more common in geriatric cats, it is necessary that these animals undergo a check-up at least twice a year in order to rule out or identify problems that could be a CKD.
Influência dos hormônios esteroides sexuais femininos na resistência insulínica em cadelas - revisão bibliográfica
(2019-12-05) Oliveira, Natália Manuela Cardoso de; Amaral, Rodrigo de Souza; http://lattes.cnpq.br/3589101457101968; Amaral, Rodrigo de Souza; http://lattes.cnpq.br/3589101457101968; Vieira, Flavia Volpato; http://lattes.cnpq.br/1627861597325307; Hoffmann, Daniel José; CV: http://lattes.cnpq.br/4603328886760993
Insulin resistance is a term that refers to the ineffective action of insulin in tissues, being developed by several factors, from infectious and nutritional diseases to the hormonal influence by endocrinopathies, use of diabetogenic drugs and physiological hormonal periods in female dogs. This review aims to describe the influence of female sex steroid hormones on the triggering of insulin resistance in non-castrated female dogs. It is observed that the mechanism of action of insulin is compromised by the action of progesterone and estradiol in the body in addition to other hyperglycemic hormones that together with genetic, environmental and autoimmune factors, can trigger transient or gestational diabetes in female dogs. It is concluded that diabetes mellitus in whole canine patients intensifies the definition of endocrinopathy as a multifactorial disorder, where there are few studies involving transient or gestational diabetes mellitus, but its risk is mentioned in the literature due to the possibility of a permanent picture in some cases. and for being responsible for conveying the importance of castration in the control of diabetic bitches and in the prevention of those at risk.
Patologias do Trato Reprodutivo de Quelônios Terrestres e de Água Doce
(2021-04-23) Merlo, Deyrdre Nunes.; Amaral, Rodrigo de Souza; Amaral, Rodrigo de Souza; Santos, Felipe Faccine dos; Chiesorin Neto, Laerzio.
Chelonians have particularities when compared to other vertebrate species, with the presence of carapace and plastron being evident. Knowledge about reproduction and reproductive pathologies that affect this group are essential due to the presence of these individuals in the veterinary clinical routine as pets and the implementation of breeding programs in captivity for endangered species. The aim of the present study was to describe the reproductive pathologies of terrestrial and freshwater turtles, addressing the main aspects of aetiology, clinical presentation, diagnosis and treatment. Among reproductive pathologies, phallus prolapse is widely reported in males, in females, dystocia and the possible complications of the lack of a treatment institution are common, in both cases the association with inadequate management is common. Less frequent reproductive diseases are also of clinical importance, since they constitute a differential diagnosis for common conditions and should not be neglected. It is noticed that many studies still need to be carried out to understand the physiopathological process of some conditions, as well as to establish prophylactic measures and better therapeutic approaches. Keywords: Reproduction. Reptiles. Diseases. Testudines. Medicine. Wild animals.
Piometra em cadelas: revisão de literatura
(2022-01-14) Nascimento, Carolayne Souza do; Amaral, Rodrigo de Souza; http://lattes.cnpq.br/8447103284183216; Vieira, Flávia Volpato; http://lattes.cnpq.br/1627861597325307; Amaral, Rodrigo de Souza; http://lattes.cnpq.br/8447103284183216; Vieira, Flavia Volpato; http://lattes.cnpq.br/1627861597325307; Ferreira, Mayara Fonseca; http://lattes.cnpq.br/2174610301180134
Pyometra is defined as a uterine infection associated with bacterial interaction that especially affects older to adult dogs. This condition is considered one of the main pathologies of the reproductive system routinely observed in clinical medicine and in small animal surgery. The canine species is more affected when compared to its incidence in felines. This happens due to the specificity of the estrous cycle of dogs in which it presents prolonged stage intervals, especially when it comes to the diestrus phase, which can last an average of sixty days. Therefore, the endometrium remains under hormonal stimulation and its modifications for a significant time. The etiopathology and mechanisms involved in the emergence of this disease are not fully elucidated by the scientific community. However, the hormonal influence during numerous reproductive cycles has been considered the main triggering agent for the development of pyometra. In addition, we can highlight that other predisposing factors are correlated to its emergence, for example: bitches with advanced age, prolonged or deregulated estrous cycles, history of pseudopregnancy and in cases of pyometra in young females, the erroneous administration of estrus inhibitors is a cause of its appearance. The objective of this work is to approach the main concepts of this pathology, as well as its etiology, clinical signs and therapeutic protocols. Keywords: estrous cycle, cystic endometrial hyperplasia, pathophysiology, reproduction
Salmonella spp. em peixes-bois da Amazônia (Trichechus inunguis) mantidos em cativeiro.
(2021-04-22) Oliveira, Bianca Cristina Rocha de; Amaral, Rodrigo de Souza; Santos, Felipe Faccini dos; Santos, Felipe Faccini dos; Tonin, Alexandre Alberto; Gouveia, Raquel
The objective was to investigate the frequency of Salmonella spp. in Amazonian manatees (Trichechus inunguis) kept in captivity at the Robin C. Best Aquatic Park located at the National Research Institute of the Amazon (LMA / Inpa). For this, analyzes were performed on fecal samples and rectal swab from 34 animals of different age groups, being nine adults, 12 puppies and 13 juveniles; in total 14 females and 20 males. The methodological process was carried out to fully characterize the strains, by isolating colonies characteristic of Salmonella spp .; identification of morphotintorial characteristics by Gram stain; identification of isolates according to the typical biochemical behavior of Salmonella spp. Triple Iron Sugar Agar (TSI), Iron Lysine Agar (LIA), Hydrogen Sulfide, Indole and Motility Agar (SIM) and Simmons Citrate Agar (CIT); confirmation of the isolates using the Polymerase Chain Reaction (PCR) technique; characterization of the sensitivity profile against the antimicrobial agents tetracyclines (30μg), ampicillin (10μg), gentamicin (10μg), cefoxitin (30μg), amoxicillin (30μg), ciprofloxacin (5μg), penicillin (10μg) and azithromycin (15μg); previous serotyping, carried out by slide agglutination tests, with specific antisera for Salmonella spp. and genetic isolation to confirm serotypes. The results of the comparison between the two collection methods were analyzed by Fisher's exact test using the GraphPad InStat program version 3.10. Of the eleven strains isolated through microbiological cultivation and biochemical characterization, nine showed positive results in PCR, confirming the positivity of three animals. The isolates came from samples collected from two young females and one juvenile female. Corresponding to a frequency of 16.6% in puppies and 7.7% in juveniles. The largest number of isolates was obtained from stool samples, however, due to the low number of positive samples, there was no significant difference between the two collection methods according to Fisher's exact test. According to the antibiogram, the isolated strains showed higher rates of efficacy for the use of tetracycline, gentamicin and ciprofloxacin. The strains were identified as Salmonella enterica subsp. salamae serotype II 42: r: - and Salmonella enterica subsp. enterica serotype Javiana. This being the first report of the occurrence of these serotypes in Amazonian manatees and the first report of Salmonella spp. in live sirenians.
Técnica de aspiração folicular guiada por ultrassom (ovum pick up – opu) em bovinos e equinos: revisão de literatura.
(2021-09-24) Gomes, Laís Almeida; Amaral, Rodrigo de Souza; Amaral, Rodrigo de Souza; Oliveira, Fernanda Amarante Mendes de; Ferreira, Mayara Fonseca
The national cattle raising obtains continuous and rapid economic development with the advancement of reproductive biotechnology techniques. These allow the possibility of increasing the production of animals that have characteristics of high interest. Ultrasound- guided follicular aspiration (Ovum Pick Up - OPU) is a widely used biotechnique in large animals with the aim of genetic improvement in the herd. This consists of obtaining oocytes from females with high added value, so that they can be used for the production of embryos in the laboratory and for the rapid dissemination of specific genetics. Mares and cows are the animals in which the technique has been most explored and has generated excellent results. The steps pertaining to the in vivo follicular aspiration technique consist of selection of oocyte donor females and prior preparation, organization of material and equipment on the day of aspiration, anesthesia and asepsis, aspiration procedure, post aspiration care, and oocyte tracking and selection collected. Most of these steps are performed in both horses and cattle. However, in view of the physiological and reproductive particularities between these two species, the steps take place in different ways. This work aims to describe the reproductive biotechnique of OPU, present the stages of the process, highlighting the methodological differences that are adopted for the use of this technique in both cattle and horses.
Tumores testiculares em cães necropsiados pelo sedivet-lpv/UFSM durante os anos de 2019 a 2021
(2022-01-14) Santos, Matheus Yuri dos; Fighera, Rafael Almeida; CV: http://lattes.cnpq.br/6223365736139655; Amaral, Rodrigo de Souza; http://lattes.cnpq.br/3589101457101968; Amaral, Rodrigo de Souza; http://lattes.cnpq.br/3589101457101968; Tonin, Alexandre Alberto; http://lattes.cnpq.br/6912106214152950; Esteves, Ísis Mônica Fontes
Reproductive system tumors are the third most frequent type of neoplasms, behind only skin and breast tumors, with testicular neoplasms commonly occurring in dogs, affecting about 10% of this population. Primary testicular tumors arise from the specialized cells that make up the organ, and include the germline germline cell tumor (seminoma), Sertoli cell tumor (sertolioma), and Leydig interstitial cell tumor (leydigoma) , being able to present age and racial predispositions. This study aimed to describe the testicular neoplasms found in necropsied dogs between the years 2019 and 2021, by the Veterinary Diagnostic Consulting Service (SediVet / LPV-UFSM), in order to characterize the morphological aspects of the lesions and their forms of occurrence and consequences, and suggest their prevalence in the study. Thus, it was observed during the period evaluated that testicular neoplasms had a low prevalence (11.5%) among necropsied dogs, being an incidental finding unrelated to the cause of death, affecting mostly elderly and geriatric individuals (75%), there is no relationship with the race. Testicular tumors occurred mostly unilaterally (70.8%), and there may be an association between tumors in the same testicle or between the testicles, which in turn may appear concomitantly with other lesions, with prostatic hyperplasia being the most observed associated finding. Keywords: Neoplasm, Testis, Multiple tumors, Reproduction.
Uso de sucedâneos lácteos na alimentação de peixes-bois em cativeiro
(2021-04-22) Oliveira, Vivian Juliana de; Amaral, Rodrigo de Souza; Sousa, Rodrigo de Souza; Mello, Daniela Magalhães Drummond; Carvalho, Vitor Luz
The aquatic mammals of the Order Sirenia are divided into two families: Trichechidae, represented by the Amazonian manatee (Trichechus inunguis), the marine manatee (T. manatus) and the African manatee (T. senegalensis), and Dugongidae , with only one representative, the dugong (Dugong dugon). The main threat to these species is the stranding of calves in the Brazilian beaches or their capture. The various factors that lead the baby to lose their mother, both marine manatee and Amazonian manatee, forced the institutions active in the rescue and rehabilitation of these animals, to develop techniques for the parental care of these animals in captivity that most resemble the mother's natural care. So far, there is no record of an ideal substitute formulation, but there are already studies using different milk formulas and good nutritional results. The description of the nutritional composition of the milk of captive marine manatees contains 17.40% of lipids, 5.25% of proteins, 125.00 mg of calcium and 105.00 mg of phosphorus. For the Amazonian manatees, variations of 8.76 and 19.73% in fat, 4.24 - 10.47% crude protein and 68.55 - 82.1% in moisture content are described. The main efficency assessment of the milk formula is based on the relation of the daily or weekly weight gain. Most of the dairy formulations described use vegetable or animal milk without lactose with the addition of some source of fat (oil or unsalted butter). Previous studies also report the importance of inserting a vitamin-mineral complex to the milk formula. Some of the formulations described have promising results in relation to the animal's weight gain and are used daily in aquatic mammal rehabilitation centers in Brazil.